Why does betadine kill bacteria

Within 15 min after the plates were inoculated, Betadine disks were distributed evenly onto the LB Agar surface, with at least 24 mm center to center between them.

The plates were incubated at 37 o C for 24 h before results were read. The diameters of the zones of complete inhibition, including the diameter of the disk, were measured to the nearest millimeter with a ruler. In addition, the remaining microorganisms on the disks were quantified by the CFU assay. All experiments were done in triplicate, and all measurements were repeated three times. The bacteria tested were Staphylococcus aureus, Pseudomonas aeruginosa, Acinetobacter baumannii, Escherichia coli, Streptococcus salivarius, Streptococcus mutans, Staphylococcus epidermidis, and Serratia marcescens.

Results : All the bacteria, except Pseudomonas aeruginosa, showed zones of inhibition against the lawns of bacteria. However, when the disks were tested for bacteria after the zone of inhibition study was completed, only three of the eight different bacteria showed killing of the bacteria on the disk.

These were, one gram negative bacteria, Serratia marcescens , and two gram positive bacteria, Streptococcus salivarius, and Streptococcus mutans.

Clinicians have used numerous strategies to combat wound infections, including topical and systemic administration of antibiotics, and various antiseptic agents such as hypochlorite bleach and hydrogen peroxide have been placed on wounds to kill bacteria or inhibit their growth.

A commonly used antimicrobial agent is povidone-iodine Betadine , a complex of iodine, the bactericidal component, with polyvinylpyrrolidone povidone , a synthetic polymer. Povidone-iodine is available as a surgical scrub or skin cleanser with a detergent base 0.

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What are antiseptics used for? Betadine TM antiseptics may be used to help manage the following wounds and skin infections: Minor cuts, wounds and abrasions — Any break in the skin caused by physical trauma such as cuts and abrasions allows the bacteria that normally reside on the skin, and other potential bacteria and fungi in the environment, to invade the tissues and cause infection.